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Quantification of CHGA + cells in ileum and nasal biopsies as determined by flow <t>cytometry.</t> Percentage of CHGA + cells within the epithelial cell population defined as EpCAM + expression in ileum tissue and nasal tissue from controls, AR patients and CRSwNP patients (n = 5/group). CHGA, chromogranin A; EpCAM: epithelial cellular adhesion molecule; AR, allergic rhinithis; CRSwNP, chronic rhinosinusitis with nasal polyps; data represented as mean ± SD, *** p < 0.005; **** p < 0.001.
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Quantification of CHGA + cells in ileum and nasal biopsies as determined by flow cytometry. Percentage of CHGA + cells within the epithelial cell population defined as EpCAM + expression in ileum tissue and nasal tissue from controls, AR patients and CRSwNP patients (n = 5/group). CHGA, chromogranin A; EpCAM: epithelial cellular adhesion molecule; AR, allergic rhinithis; CRSwNP, chronic rhinosinusitis with nasal polyps; data represented as mean ± SD, *** p < 0.005; **** p < 0.001.

Journal: Frontiers in Immunology

Article Title: Rare presence and function of neuroendocrine cells in the nasal mucosa

doi: 10.3389/fimmu.2024.1394539

Figure Lengend Snippet: Quantification of CHGA + cells in ileum and nasal biopsies as determined by flow cytometry. Percentage of CHGA + cells within the epithelial cell population defined as EpCAM + expression in ileum tissue and nasal tissue from controls, AR patients and CRSwNP patients (n = 5/group). CHGA, chromogranin A; EpCAM: epithelial cellular adhesion molecule; AR, allergic rhinithis; CRSwNP, chronic rhinosinusitis with nasal polyps; data represented as mean ± SD, *** p < 0.005; **** p < 0.001.

Article Snippet: Sequentially, intracellular staining was performed using Flow Cytometry Fixation & permeabilization Buffer Kit I (FC009, Biotechne) with 50 µl/ml PE Anti-Human Chromogranin A Clone S21-537 (RUO) antibody (5564563, BD Pharmingen).

Techniques: Flow Cytometry, Expressing